Development and validation of an automated liquid-liquid extraction GC/MS method for the determination of THC, 11-OH-THC, and free THC-carboxylic acid (THC-COOH) from blood serum
نویسندگان
چکیده
منابع مشابه
Development and validation of an automated liquid-liquid extraction GC/MS method for the determination of THC, 11-OH-THC, and free THC-carboxylic acid (THC-COOH) from blood serum
The analysis of Δ(9)-tetrahydrocannabinol (THC) and its metabolites 11-hydroxy-Δ(9)-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol (THC-COOH) from blood serum is a routine task in forensic toxicology laboratories. For examination of consumption habits, the concentration of the phase I metabolite THC-COOH is used. Recommendations for interpretation of analysis v...
متن کاملNew extraction method of THC and its metabolites, 11-OH-THC and THC-COOH, in plasma
Objectives: A liquid/liquid extraction technique on solid support of Δ9-tetradydrocannabinol (THC), 11-hydroxy-Δ9-tetradydrocannabinol (11-OH-THC) and 11-nor-Δ9-tetradydrocannabinol-9-carboxylic acid (THCCOOH) in plasma was developed in order to be assayed by high-performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Methods: The samples were extracted by liquid/liquid ex...
متن کاملNovel automated extraction method for quantitative analysis of urinary 11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH).
An automated extraction method for extracting the major urinary metabolite of cannabis, 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) was developed on the four-probe Gilson ASPEC XL4trade mark solid-phase extraction (SPE) system. The method works on liquid-liquid extraction principles but does not require the use of SPE cartridges. The limits of detection and quantitation an...
متن کاملSimultaneous and sensitive analysis of THC, 11-OH-THC, THC-COOH, CBD, and CBN by GC-MS in plasma after oral application of small doses of THC and cannabis extract.
Besides the psychoactive Delta(9)-tetrahydrocannabinol (THC), hashish and marijuana as well as cannabis-based medicine extracts contain varying amounts of cannabidiol (CBD) and of the degradation product cannabinol (CBN). The additional determination of these compounds is interesting from forensic and medical points of view because it can be used for further proof of cannabis exposure and becau...
متن کاملSolid-phase extraction and analysis of THC and carboxy-THC from whole blood using a novel fluorinated solid-phase extraction sorbent and fast liquid chromatography-tandem mass spectrometry.
In this study, solid-phase extraction (SPE) is described using a novel fluorinated [heptadecafluorotetrahydrodecyl (C(10)H(4)F(17))] phase to isolate THC and its primary metabolite carboxy-THC from whole blood samples. SPE was performed in hydrophobic mode after samples of whole blood were precipitated with acetonitrile. After applying the sample to the SPE column in aqueous phosphate buffer (p...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2016
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-016-9537-5